Hari B. Krishnan and colleagues explain that the substance, Bowman-Birk Protease Inhibitor (BBI),

They found that soybean seeds incubated in water at 122 degrees Fahrenheit naturally release large amounts of BBI that can easily be harvested from the water. The protein appeared to be active, with tests showing that it stopped breast cancer cells from dividing in a laboratory dish. “The abundance of BBI in soybean seed exudates by incubating the seeds in warm water provides a simple and alternative method to isolate this low molecular weight protein,” the researchers said.
The scientists acknowledge funding from the Agricultural Research Service of the U.S. Department of Agriculture.
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